type: position_score
table:
  filename: ENCFF731RBX.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: TF_ChIP-seq_ENCSR681KXT
  column_index: 3
  type: float
  desc: signal
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'ChIP-seq ENCSR681KXT [TISSUE: Homo sapiens HepG2 genetically modified
    (insertion) using CRISPR targeting H. sapiens ZNF329, TARGET: ZNF329]'
  description: '**status**: released


    **biological\_replicates**: Rep 1, Rep 2


    **summary**: genetically modified (insertion) using CRISPR targeting H. sapiens
    ZNF329


    **output\_type**: IDR thresholded peaks


    **audit\_internal\_action**: Released analysis {ENCAN466XDH|/analyses/ENCAN466XDH/}
    has in progress subobject quality standard {encode4-tf-chip|/quality-standards/encode4-tf-chip/}


    **audit\_warning**: Processed alignments file {ENCFF830AUJ|/files/ENCFF830AUJ/}
    processed by ChIP-seq ENCODE4 v1.9.0 GRCh38 pipeline has 19504989 usable fragments.
    The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting
    ZNF329-human and investigated as a transcription factor is 10 million usable fragments.
    The recommended value is > 20 million, but > 10 million is acceptable. (See {ENCODE
    ChIP-seq data standards|/data-standards/chip-seq/} )


    **audit\_warning**: Processed alignments file {ENCFF674HEV|/files/ENCFF674HEV/}
    processed by ChIP-seq ENCODE4 v1.9.0 GRCh38 pipeline has 13352555 usable fragments.
    The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting
    ZNF329-human and investigated as a transcription factor is 10 million usable fragments.
    The recommended value is > 20 million, but > 10 million is acceptable. (See {ENCODE
    ChIP-seq data standards|/data-standards/chip-seq/} )


    **audit\_warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\_distinct) is
    the ratio of the number of genomic locations where exactly one read maps uniquely
    (M1) to the number of genomic locations where some reads map (M\_distinct). A
    PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate
    bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking.
    PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments
    file {ENCFF830AUJ|/files/ENCFF830AUJ/} processed by ChIP-seq ENCODE4 v1.9.0 GRCh38
    pipeline was generated from a library with PBC1 value of 0.88.


    **audit\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio
    of the number of genomic locations where exactly one read maps uniquely (M1) to
    the number of genomic locations where two reads map uniquely (M2). A PBC2 value
    in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3
    - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,
    but > 3 is acceptable. ENCODE processed alignments file {ENCFF830AUJ|/files/ENCFF830AUJ/}
    processed by ChIP-seq ENCODE4 v1.9.0 GRCh38 pipeline was generated from a library
    with PBC2 value of 8.21.


    **audit\_warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\_distinct) is
    the ratio of the number of genomic locations where exactly one read maps uniquely
    (M1) to the number of genomic locations where some reads map (M\_distinct). A
    PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate
    bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking.
    PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments
    file {ENCFF674HEV|/files/ENCFF674HEV/} processed by ChIP-seq ENCODE4 v1.9.0 GRCh38
    pipeline was generated from a library with PBC1 value of 0.90.


    **audit\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio
    of the number of genomic locations where exactly one read maps uniquely (M1) to
    the number of genomic locations where two reads map uniquely (M2). A PBC2 value
    in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3
    - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,
    but > 3 is acceptable. ENCODE processed alignments file {ENCFF674HEV|/files/ENCFF674HEV/}
    processed by ChIP-seq ENCODE4 v1.9.0 GRCh38 pipeline was generated from a library
    with PBC2 value of 9.60.'
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR681KXT
    status: released
    assay_term_name: ChIP-seq
    simple_biosample_summary: genetically modified (insertion) using CRISPR targeting
      H. sapiens ZNF329
    biosample_summary: Homo sapiens HepG2 genetically modified (insertion) using CRISPR
      targeting H. sapiens ZNF329
    replication_type: isogenic
    biosample_ontology: /biosample-types/cell_line_EFO_0001187/
    perturbed: false
    doi: 10.17989/ENCSR681KXT
    date_created: '2021-05-18T18:07:07.785268+00:00'
    date_released: '2021-08-13'
    submitter_comment: ''
    target: ZNF329