type: position_score
table:
  filename: ENCFF807XMX.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: TF_ChIP-seq_ENCSR179SAO
  column_index: 3
  type: float
  desc: 'TF_ChIP-seq ENCSR179SAO [biosample_summary="Homo sapiens A673" and target="EZH2"]'
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'TF_ChIP-seq ENCSR179SAO [biosample_summary="Homo sapiens A673" and target="EZH2"]'
  description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\
    \n**summary**: \n\n**output\\_type**: optimal IDR thresholded peaks\n\n**audit\\\
    _internal\\_action**: Released analysis {ENCAN827SHR|/analyses/ENCAN827SHR/} has\
    \ in progress subobject quality standard {encode3-tf-chip|/quality-standards/encode3-tf-chip/}\n\
    \n**audit\\_internal\\_action**: File {ENCFF807XMX|/files/ENCFF807XMX/} with status\
    \ 'released' is derived from file {ENCFF419RSJ|/files/ENCFF419RSJ/} with status\
    \ 'archived'.\n\n**audit\\_not\\_compliant**: NRF (Non Redundant Fraction) is\
    \ equal to the result of the division of the number of reads after duplicates\
    \ removal by the total number of reads. An NRF value in the range 0 - 0.5 is poor\
    \ complexity, 0.5 - 0.8 is moderate complexity, and > 0.8 high complexity. NRF\
    \ value > 0.8 is recommended, but > 0.5 is acceptable. ENCODE processed alignments\
    \ file {ENCFF497AWS|/files/ENCFF497AWS/} processed by ChIP-seq ENCODE3 GRCh38\
    \ pipeline was generated from a library with NRF value of 0.39.\n\n**audit\\_not\\\
    _compliant**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\\_distinct) is the ratio\
    \ of the number of genomic locations where exactly one read maps uniquely (M1)\
    \ to the number of genomic locations where some reads map (M\\_distinct). A PBC1\
    \ value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate bottlenecking,\
    \ 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking. PBC1 value >\
    \ 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments file\
    \ {ENCFF497AWS|/files/ENCFF497AWS/} processed by ChIP-seq ENCODE3 GRCh38 pipeline\
    \ was generated from a library with PBC1 value of 0.37.\n\n**audit\\_warning**:\
    \ Processed alignments file {ENCFF497AWS|/files/ENCFF497AWS/} processed by ChIP-seq\
    \ ENCODE3 GRCh38 pipeline has 15194282 usable fragments. The minimum ENCODE standard\
    \ for each replicate in a ChIP-seq experiment targeting EZH2-human and investigated\
    \ as a transcription factor is 10 million usable fragments. The recommended value\
    \ is > 20 million, but > 10 million is acceptable. (See {ENCODE ChIP-seq data\
    \ standards|/data-standards/chip-seq/} )\n\n**audit\\_warning**: PBC2 (PCR Bottlenecking\
    \ Coefficient 2, M1/M2) is the ratio of the number of genomic locations where\
    \ exactly one read maps uniquely (M1) to the number of genomic locations where\
    \ two reads map uniquely (M2). A PBC2 value in the range 0 - 1 is severe bottlenecking,\
    \ 1 - 3 is moderate bottlenecking, 3 - 10 is mild bottlenecking, > 10 is no bottlenecking.\
    \ PBC2 value > 10 is recommended, but > 3 is acceptable. ENCODE processed alignments\
    \ file {ENCFF497AWS|/files/ENCFF497AWS/} processed by ChIP-seq ENCODE3 GRCh38\
    \ pipeline was generated from a library with PBC2 value of 1.70.\n\n**audit\\\
    _warning**: Replicate concordance in ChIP-seq experiments is measured by calculating\
    \ IDR values (Irreproducible Discovery Rate). ENCODE processed IDR thresholded\
    \ peaks files {ENCFF227DYU|/files/ENCFF227DYU/}, {ENCFF790MVL|/files/ENCFF790MVL/}\
    \ processed by ChIP-seq ENCODE3 GRCh38 pipeline have a rescue ratio of 1.45 and\
    \ a self consistency ratio of 3.55. According to ENCODE standards, having both\
    \ rescue ratio and self consistency ratio values < 2 is recommended, but having\
    \ only one of the ratio values < 2 is acceptable.\n\n**audit\\_warning**: Replicate\
    \ concordance in ChIP-seq experiments is measured by calculating IDR values (Irreproducible\
    \ Discovery Rate). ENCODE processed IDR thresholded peaks files {ENCFF528EBQ|/files/ENCFF528EBQ/},\
    \ {ENCFF807XMX|/files/ENCFF807XMX/} processed by ChIP-seq ENCODE3 GRCh38 pipeline\
    \ have a rescue ratio of 1.45 and a self consistency ratio of 3.55. According\
    \ to ENCODE standards, having both rescue ratio and self consistency ratio values\
    \ < 2 is recommended, but having only one of the ratio values < 2 is acceptable."
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR179SAO
    status: released
    assay_term_name: ChIP-seq
    simple_biosample_summary: ''
    biosample_summary: Homo sapiens A673
    replication_type: isogenic
    biosample_ontology: /biosample-types/cell_line_EFO_0002106/
    perturbed: false
    doi: 10.17989/ENCSR179SAO
    date_created: '2015-05-20T20:37:12.478952+00:00'
    date_released: '2017-11-29'
    submitter_comment: ''
    target: EZH2