type: position_score table: filename: ENCFF521WMG.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR960ZMM column_index: 3 type: float desc: 'Histone_ChIP-seq ENCSR960ZMM [biosample_summary="Homo sapiens OCI-LY1" and target="H4K20me1"]' histogram: type: number number_of_bins: 100 meta: summary: 'Histone_ChIP-seq ENCSR960ZMM [biosample_summary="Homo sapiens OCI-LY1" and target="H4K20me1"]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\ \n**summary**: \n\n**output\\_type**: replicated peaks\n\n**audit\\_internal\\\ _action**: Archived analysis {ENCAN902AXY|/analyses/ENCAN902AXY/} has in progress\ \ subobject quality standard {encode3-histone-chip|/quality-standards/encode3-histone-chip/}\n\ \n**audit\\_not\\_compliant**: Processed alignments file {ENCFF729YBU|/files/ENCFF729YBU/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline has 25188316 usable fragments. The\ \ minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H4K20me1-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\\_distinct) is the ratio\ \ of the number of genomic locations where exactly one read maps uniquely (M1)\ \ to the number of genomic locations where some reads map (M\\_distinct). A PBC1\ \ value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate bottlenecking,\ \ 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking. PBC1 value >\ \ 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments file\ \ {ENCFF454HDW|/files/ENCFF454HDW/} processed by ChIP-seq ENCODE3 hg19 pipeline\ \ was generated from a library with PBC1 value of 0.87.\n\n**audit\\_warning**:\ \ PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio of the number of\ \ genomic locations where exactly one read maps uniquely (M1) to the number of\ \ genomic locations where two reads map uniquely (M2). A PBC2 value in the range\ \ 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3 - 10 is mild\ \ bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended, but\ \ > 3 is acceptable. ENCODE processed alignments file {ENCFF454HDW|/files/ENCFF454HDW/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline was generated from a library with\ \ PBC2 value of 7.65." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR960ZMM status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens OCI-LY1 replication_type: isogenic biosample_ontology: /biosample-types/cell_line_EFO_0005907/ perturbed: false doi: 10.17989/ENCSR960ZMM date_created: '2014-02-20T04:37:52.882481+00:00' date_released: '2016-10-17' submitter_comment: '' target: H4K20me1