type: position_score table: filename: ENCFF483KOI.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR879WTY column_index: 3 type: float desc: 'Histone_ChIP-seq ENCSR879WTY [biosample_summary="Homo sapiens KOPT-K1" and target="H3K36me3"]' histogram: type: number number_of_bins: 100 meta: summary: 'Histone_ChIP-seq ENCSR879WTY [biosample_summary="Homo sapiens KOPT-K1" and target="H3K36me3"]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\ \n**summary**: \n\n**output\\_type**: replicated peaks\n\n**audit\\_internal\\\ _action**: Archived analysis {ENCAN831ROI|/analyses/ENCAN831ROI/} has in progress\ \ subobject quality standard {encode3-histone-chip|/quality-standards/encode3-histone-chip/}\n\ \n**audit\\_not\\_compliant**: Processed alignments file {ENCFF998CRR|/files/ENCFF998CRR/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline has 9351237 usable fragments. The\ \ minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K36me3-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _not\\_compliant**: Processed alignments file {ENCFF669JSW|/files/ENCFF669JSW/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline has 18956684 usable fragments. The\ \ minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K36me3-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _warning**: NRF (Non Redundant Fraction) is equal to the result of the division\ \ of the number of reads after duplicates removal by the total number of reads.\ \ An NRF value in the range 0 - 0.5 is poor complexity, 0.5 - 0.8 is moderate\ \ complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended, but >\ \ 0.5 is acceptable. ENCODE processed alignments file {ENCFF998CRR|/files/ENCFF998CRR/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline was generated from a library with\ \ NRF value of 0.56.\n\n**audit\\_warning**: PBC1 (PCR Bottlenecking Coefficient\ \ 1, M1/M\\_distinct) is the ratio of the number of genomic locations where exactly\ \ one read maps uniquely (M1) to the number of genomic locations where some reads\ \ map (M\\_distinct). A PBC1 value in the range 0 - 0.5 is severe bottlenecking,\ \ 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking, and >\ \ 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is acceptable.\ \ ENCODE processed alignments file {ENCFF998CRR|/files/ENCFF998CRR/} processed\ \ by ChIP-seq ENCODE3 hg19 pipeline was generated from a library with PBC1 value\ \ of 0.55.\n\n**audit\\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2)\ \ is the ratio of the number of genomic locations where exactly one read maps\ \ uniquely (M1) to the number of genomic locations where two reads map uniquely\ \ (M2). A PBC2 value in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate\ \ bottlenecking, 3 - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2\ \ value > 10 is recommended, but > 3 is acceptable. ENCODE processed alignments\ \ file {ENCFF998CRR|/files/ENCFF998CRR/} processed by ChIP-seq ENCODE3 hg19 pipeline\ \ was generated from a library with PBC2 value of 2.16." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR879WTY status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens KOPT-K1 replication_type: isogenic biosample_ontology: /biosample-types/cell_line_EFO_0006283/ perturbed: false doi: 10.17989/ENCSR879WTY date_created: '2014-10-24T16:18:20.931311+00:00' date_released: '2017-01-26' submitter_comment: '' target: H3K36me3