type: position_score table: filename: ENCFF416JWX.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR870IGD column_index: 3 type: float desc: 'Histone_ChIP-seq ENCSR870IGD [biosample_summary="Homo sapiens Karpas-422" and target="H3K4me2"]' histogram: type: number number_of_bins: 100 meta: summary: 'Histone_ChIP-seq ENCSR870IGD [biosample_summary="Homo sapiens Karpas-422" and target="H3K4me2"]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\ \n**summary**: \n\n**output\\_type**: pseudoreplicated peaks\n\n**audit\\_internal\\\ _action**: Released analysis {ENCAN494NLO|/analyses/ENCAN494NLO/} has in progress\ \ subobject document {e35c26dc-8ff4-44c4-9225-cf154828d0e3|/documents/e35c26dc-8ff4-44c4-9225-cf154828d0e3/}\n\ \n**audit\\_internal\\_action**: Released analysis {ENCAN494NLO|/analyses/ENCAN494NLO/}\ \ has in progress subobject quality standard {encode4-histone-chip|/quality-standards/encode4-histone-chip/}\n\ \n**audit\\_not\\_compliant**: NRF (Non Redundant Fraction) is equal to the result\ \ of the division of the number of reads after duplicates removal by the total\ \ number of reads. An NRF value in the range 0 - 0.5 is poor complexity, 0.5 -\ \ 0.8 is moderate complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended,\ \ but > 0.5 is acceptable. ENCODE processed alignments file {ENCFF388OOO|/files/ENCFF388OOO/}\ \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\ \ with NRF value of 0.45.\n\n**audit\\_not\\_compliant**: PBC1 (PCR Bottlenecking\ \ Coefficient 1, M1/M\\_distinct) is the ratio of the number of genomic locations\ \ where exactly one read maps uniquely (M1) to the number of genomic locations\ \ where some reads map (M\\_distinct). A PBC1 value in the range 0 - 0.5 is severe\ \ bottlenecking, 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking,\ \ and > 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is\ \ acceptable. ENCODE processed alignments file {ENCFF388OOO|/files/ENCFF388OOO/}\ \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\ \ with PBC1 value of 0.41.\n\n**audit\\_warning**: Processed alignments file {ENCFF388OOO|/files/ENCFF388OOO/}\ \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline has 12118747 usable fragments.\ \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K4me2-human and investigated as a narrow histone mark is 10 million usable\ \ fragments. The recommended value is > 20 million, but > 10 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio of the\ \ number of genomic locations where exactly one read maps uniquely (M1) to the\ \ number of genomic locations where two reads map uniquely (M2). A PBC2 value\ \ in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking,\ \ 3 - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,\ \ but > 3 is acceptable. ENCODE processed alignments file {ENCFF388OOO|/files/ENCFF388OOO/}\ \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\ \ with PBC2 value of 1.56." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR870IGD status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens Karpas-422 replication_type: isogenic biosample_ontology: /biosample-types/cell_line_EFO_0005719/ perturbed: false doi: 10.17989/ENCSR870IGD date_created: '2014-02-20T05:41:58.275461+00:00' date_released: '2014-07-11' submitter_comment: '' target: H3K4me2