type: position_score table: filename: ENCFF427YQG.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR807WEO column_index: 3 type: float desc: signal histogram: type: number number_of_bins: 100 meta: summary: 'ChIP-seq ENCSR807WEO [TISSUE: Homo sapiens CD4-positive, alpha-beta memory T cell originated from blood cell, TARGET: H3K4me3]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1\n\n**summary**:\ \ \n\n**output\\_type**: pseudoreplicated peaks\n\n**audit\\_error**: Processed\ \ redacted alignments file {ENCFF401LNB|/files/ENCFF401LNB/} processed by ChIP-seq\ \ ENCODE3 GRCh38 pipeline has 4907286 usable fragments. The minimum ENCODE standard\ \ for each replicate in a ChIP-seq experiment targeting H3K4me3-human and investigated\ \ as a narrow histone mark is 10 million usable fragments. The recommended value\ \ is > 20 million, but > 10 million is acceptable. (See {ENCODE ChIP-seq data\ \ standards|/data-standards/chip-seq/})\n\n**audit\\_internal\\_action**: Archived\ \ analysis {ENCAN373APQ|/analyses/ENCAN373APQ/} has in progress subobject quality\ \ standard {encode3-histone-chip|/quality-standards/encode3-histone-chip/}\n\n\ **audit\\_not\\_compliant**: NRF (Non Redundant Fraction) is equal to the result\ \ of the division of the number of reads after duplicates removal by the total\ \ number of reads. An NRF value in the range 0 - 0.5 is poor complexity, 0.5 -\ \ 0.8 is moderate complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended,\ \ but > 0.5 is acceptable. ENCODE processed redacted alignments file {ENCFF401LNB|/files/ENCFF401LNB/}\ \ processed by ChIP-seq ENCODE3 GRCh38 pipeline was generated from a library with\ \ NRF value of 0.32.\n\n**audit\\_not\\_compliant**: PBC1 (PCR Bottlenecking Coefficient\ \ 1, M1/M\\_distinct) is the ratio of the number of genomic locations where exactly\ \ one read maps uniquely (M1) to the number of genomic locations where some reads\ \ map (M\\_distinct). A PBC1 value in the range 0 - 0.5 is severe bottlenecking,\ \ 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking, and >\ \ 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is acceptable.\ \ ENCODE processed redacted alignments file {ENCFF401LNB|/files/ENCFF401LNB/}\ \ processed by ChIP-seq ENCODE3 GRCh38 pipeline was generated from a library with\ \ PBC1 value of 0.32.\n\n**audit\\_warning**: PBC2 (PCR Bottlenecking Coefficient\ \ 2, M1/M2) is the ratio of the number of genomic locations where exactly one\ \ read maps uniquely (M1) to the number of genomic locations where two reads map\ \ uniquely (M2). A PBC2 value in the range 0 - 1 is severe bottlenecking, 1 -\ \ 3 is moderate bottlenecking, 3 - 10 is mild bottlenecking, > 10 is no bottlenecking.\ \ PBC2 value > 10 is recommended, but > 3 is acceptable. ENCODE processed redacted\ \ alignments file {ENCFF401LNB|/files/ENCFF401LNB/} processed by ChIP-seq ENCODE3\ \ GRCh38 pipeline was generated from a library with PBC2 value of 1.83." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR807WEO status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens CD4-positive, alpha-beta memory T cell originated from blood cell replication_type: unreplicated biosample_ontology: /biosample-types/primary_cell_CL_0000897/ perturbed: false doi: 10.17989/ENCSR807WEO date_created: '2015-09-15T19:49:05.833646+00:00' date_released: '2013-07-31' submitter_comment: '' target: H3K4me3