type: position_score table: filename: ENCFF775AXL.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR797GOJ column_index: 3 type: float desc: 'Histone_ChIP-seq ENCSR797GOJ [biosample_summary="Homo sapiens CD8-positive, alpha-beta T cell" and target="H3K27me3"]' histogram: type: number number_of_bins: 100 meta: summary: 'Histone_ChIP-seq ENCSR797GOJ [biosample_summary="Homo sapiens CD8-positive, alpha-beta T cell" and target="H3K27me3"]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\ \n**summary**: \n\n**output\\_type**: replicated peaks\n\n**audit\\_internal\\\ _action**: Archived analysis {ENCAN993VOW|/analyses/ENCAN993VOW/} has in progress\ \ subobject quality standard {encode3-histone-chip|/quality-standards/encode3-histone-chip/}\n\ \n**audit\\_not\\_compliant**: Processed redacted alignments file {ENCFF504BFM|/files/ENCFF504BFM/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline has 13041431 usable fragments. The\ \ minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K27me3-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _not\\_compliant**: Processed redacted alignments file {ENCFF955QEC|/files/ENCFF955QEC/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline has 14663363 usable fragments. The\ \ minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K27me3-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\\_distinct) is the ratio\ \ of the number of genomic locations where exactly one read maps uniquely (M1)\ \ to the number of genomic locations where some reads map (M\\_distinct). A PBC1\ \ value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate bottlenecking,\ \ 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking. PBC1 value >\ \ 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed redacted alignments\ \ file {ENCFF955QEC|/files/ENCFF955QEC/} processed by ChIP-seq ENCODE3 hg19 pipeline\ \ was generated from a library with PBC1 value of 0.86.\n\n**audit\\_warning**:\ \ PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio of the number of\ \ genomic locations where exactly one read maps uniquely (M1) to the number of\ \ genomic locations where two reads map uniquely (M2). A PBC2 value in the range\ \ 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3 - 10 is mild\ \ bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended, but\ \ > 3 is acceptable. ENCODE processed redacted alignments file {ENCFF955QEC|/files/ENCFF955QEC/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline was generated from a library with\ \ PBC2 value of 6.93." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR797GOJ status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens CD8-positive, alpha-beta T cell replication_type: anisogenic biosample_ontology: /biosample-types/primary_cell_CL_0000625/ perturbed: false doi: 10.17989/ENCSR797GOJ date_created: '2015-09-15T19:48:44.512008+00:00' date_released: '2013-07-31' submitter_comment: Experiment 90a3a661-07db-4d9d-8c86-a047162e9746 merged into base. target: H3K27me3