type: position_score table: filename: ENCFF409FRO.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR750ZCV column_index: 3 type: float desc: signal histogram: type: number number_of_bins: 100 meta: summary: 'ChIP-seq ENCSR750ZCV [TISSUE: Homo sapiens effector memory CD4-positive, alpha-beta T cell, TARGET: H3K9me3]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\ \n**summary**: \n\n**output\\_type**: replicated peaks\n\n**audit\\_internal\\\ _action**: Archived analysis {ENCAN463AGR|/analyses/ENCAN463AGR/} has in progress\ \ subobject quality standard {encode3-histone-chip|/quality-standards/encode3-histone-chip/}\n\ \n**audit\\_not\\_compliant**: Processed unfiltered alignments file {ENCFF370HOZ|/files/ENCFF370HOZ/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline has 16612401 mapped reads. The minimum\ \ ENCODE standard for each replicate in a ChIP-seq experiment targeting H3K9me3-human\ \ and investigated as a broad histone mark is 35 million mapped reads. The recommended\ \ value is > 45 million, but > 35 million is acceptable. (See {ENCODE ChIP-seq\ \ data standards|/data-standards/chip-seq/})\n\n**audit\\_not\\_compliant**: Processed\ \ redacted unfiltered alignments file {ENCFF831ZRN|/files/ENCFF831ZRN/} processed\ \ by ChIP-seq ENCODE3 hg19 pipeline has 25310046 mapped reads. The minimum ENCODE\ \ standard for each replicate in a ChIP-seq experiment targeting H3K9me3-human\ \ and investigated as a broad histone mark is 35 million mapped reads. The recommended\ \ value is > 45 million, but > 35 million is acceptable. (See {ENCODE ChIP-seq\ \ data standards|/data-standards/chip-seq/})\n\n**audit\\_warning**: NRF (Non\ \ Redundant Fraction) is equal to the result of the division of the number of\ \ reads after duplicates removal by the total number of reads. An NRF value in\ \ the range 0 - 0.5 is poor complexity, 0.5 - 0.8 is moderate complexity, and\ \ > 0.8 high complexity. NRF value > 0.8 is recommended, but > 0.5 is acceptable.\ \ ENCODE processed redacted alignments file {ENCFF742SNS|/files/ENCFF742SNS/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline was generated from a library with\ \ NRF value of 0.73.\n\n**audit\\_warning**: PBC1 (PCR Bottlenecking Coefficient\ \ 1, M1/M\\_distinct) is the ratio of the number of genomic locations where exactly\ \ one read maps uniquely (M1) to the number of genomic locations where some reads\ \ map (M\\_distinct). A PBC1 value in the range 0 - 0.5 is severe bottlenecking,\ \ 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking, and >\ \ 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is acceptable.\ \ ENCODE processed redacted alignments file {ENCFF742SNS|/files/ENCFF742SNS/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline was generated from a library with\ \ PBC1 value of 0.73.\n\n**audit\\_warning**: PBC2 (PCR Bottlenecking Coefficient\ \ 2, M1/M2) is the ratio of the number of genomic locations where exactly one\ \ read maps uniquely (M1) to the number of genomic locations where two reads map\ \ uniquely (M2). A PBC2 value in the range 0 - 1 is severe bottlenecking, 1 -\ \ 3 is moderate bottlenecking, 3 - 10 is mild bottlenecking, > 10 is no bottlenecking.\ \ PBC2 value > 10 is recommended, but > 3 is acceptable. ENCODE processed redacted\ \ alignments file {ENCFF742SNS|/files/ENCFF742SNS/} processed by ChIP-seq ENCODE3\ \ hg19 pipeline was generated from a library with PBC2 value of 3.58." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR750ZCV status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens effector memory CD4-positive, alpha-beta T cell replication_type: anisogenic biosample_ontology: /biosample-types/primary_cell_CL_0000905/ perturbed: false doi: 10.17989/ENCSR750ZCV date_created: '2015-09-15T19:49:06.534554+00:00' date_released: '2013-07-31' submitter_comment: The raw data from at least one replicate is not available to the ENCODE consortium. target: H3K9me3