type: position_score
table:
  filename: ENCFF723NVE.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: Histone_ChIP-seq_ENCSR581PUR
  column_index: 3
  type: float
  desc: signal
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'ChIP-seq ENCSR581PUR [TISSUE: Homo sapiens A673, TARGET: H3K36me3]'
  description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\
    \n**summary**: \n\n**output\\_type**: pseudoreplicated peaks\n\n**audit\\_internal\\\
    _action**: Released analysis {ENCAN400KKI|/analyses/ENCAN400KKI/} has in progress\
    \ subobject quality standard {encode4-histone-chip|/quality-standards/encode4-histone-chip/}\n\
    \n**audit\\_internal\\_action**: Released analysis {ENCAN400KKI|/analyses/ENCAN400KKI/}\
    \ has in progress subobject document {fe9e7a6f-0f1e-4a7a-9d92-52692503c8a5|/documents/fe9e7a6f-0f1e-4a7a-9d92-52692503c8a5/}\n\
    \n**audit\\_not\\_compliant**: Processed alignments file {ENCFF575JXR|/files/ENCFF575JXR/}\
    \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline has 23378004 usable fragments.\
    \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\
    \ H3K36me3-human and investigated as a broad histone mark is 20 million usable\
    \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\
    \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\
    _not\\_compliant**: Processed alignments file {ENCFF145ZRI|/files/ENCFF145ZRI/}\
    \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline has 7674338 usable fragments.\
    \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\
    \ H3K36me3-human and investigated as a broad histone mark is 20 million usable\
    \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\
    \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\
    _not\\_compliant**: NRF (Non Redundant Fraction) is equal to the result of the\
    \ division of the number of reads after duplicates removal by the total number\
    \ of reads. An NRF value in the range 0 - 0.5 is poor complexity, 0.5 - 0.8 is\
    \ moderate complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended,\
    \ but > 0.5 is acceptable. ENCODE processed alignments file {ENCFF145ZRI|/files/ENCFF145ZRI/}\
    \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\
    \ with NRF value of 0.23.\n\n**audit\\_not\\_compliant**: PBC1 (PCR Bottlenecking\
    \ Coefficient 1, M1/M\\_distinct) is the ratio of the number of genomic locations\
    \ where exactly one read maps uniquely (M1) to the number of genomic locations\
    \ where some reads map (M\\_distinct). A PBC1 value in the range 0 - 0.5 is severe\
    \ bottlenecking, 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking,\
    \ and > 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is\
    \ acceptable. ENCODE processed alignments file {ENCFF145ZRI|/files/ENCFF145ZRI/}\
    \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\
    \ with PBC1 value of 0.19.\n\n**audit\\_warning**: PBC2 (PCR Bottlenecking Coefficient\
    \ 2, M1/M2) is the ratio of the number of genomic locations where exactly one\
    \ read maps uniquely (M1) to the number of genomic locations where two reads map\
    \ uniquely (M2). A PBC2 value in the range 0 - 1 is severe bottlenecking, 1 -\
    \ 3 is moderate bottlenecking, 3 - 10 is mild bottlenecking, > 10 is no bottlenecking.\
    \ PBC2 value > 10 is recommended, but > 3 is acceptable. ENCODE processed alignments\
    \ file {ENCFF145ZRI|/files/ENCFF145ZRI/} processed by ChIP-seq ENCODE4 v1.6.1\
    \ GRCh38 pipeline was generated from a library with PBC2 value of 1.31."
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR581PUR
    status: released
    assay_term_name: ChIP-seq
    simple_biosample_summary: ''
    biosample_summary: Homo sapiens A673
    replication_type: isogenic
    biosample_ontology: /biosample-types/cell_line_EFO_0002106/
    perturbed: false
    doi: 10.17989/ENCSR581PUR
    date_created: '2015-05-20T20:38:54.363820+00:00'
    date_released: '2016-05-06'
    submitter_comment: Files ENCFF438MMK, ENCFF213GKU had incorrectly identified paired_end
      1 and 2 metadata respectively. They have been corrected to match its fastq file
      flowcell signature accordingly.
    target: H3K36me3