type: position_score table: filename: ENCFF976PQR.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR580WRV column_index: 3 type: float desc: signal histogram: type: number number_of_bins: 100 meta: summary: 'ChIP-seq ENCSR580WRV [TISSUE: Homo sapiens CD4-positive, alpha-beta memory T cell, TARGET: H3K36me3]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\ \n**summary**: \n\n**output\\_type**: pseudoreplicated peaks\n\n**audit\\_internal\\\ _action**: Released analysis {ENCAN886XSY|/analyses/ENCAN886XSY/} has in progress\ \ subobject quality standard {encode4-histone-chip|/quality-standards/encode4-histone-chip/}\n\ \n**audit\\_not\\_compliant**: Processed redacted alignments file {ENCFF013ZAX|/files/ENCFF013ZAX/}\ \ processed by ChIP-seq ENCODE4 v1.7.0 GRCh38 pipeline has 26161508 usable fragments.\ \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K36me3-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _not\\_compliant**: Processed redacted alignments file {ENCFF489CFC|/files/ENCFF489CFC/}\ \ processed by ChIP-seq ENCODE4 v1.7.0 GRCh38 pipeline has 30233360 usable fragments.\ \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K36me3-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _warning**: NRF (Non Redundant Fraction) is equal to the result of the division\ \ of the number of reads after duplicates removal by the total number of reads.\ \ An NRF value in the range 0 - 0.5 is poor complexity, 0.5 - 0.8 is moderate\ \ complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended, but >\ \ 0.5 is acceptable. ENCODE processed redacted alignments file {ENCFF489CFC|/files/ENCFF489CFC/}\ \ processed by ChIP-seq ENCODE4 v1.7.0 GRCh38 pipeline was generated from a library\ \ with NRF value of 0.78.\n\n**audit\\_warning**: PBC1 (PCR Bottlenecking Coefficient\ \ 1, M1/M\\_distinct) is the ratio of the number of genomic locations where exactly\ \ one read maps uniquely (M1) to the number of genomic locations where some reads\ \ map (M\\_distinct). A PBC1 value in the range 0 - 0.5 is severe bottlenecking,\ \ 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking, and >\ \ 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is acceptable.\ \ ENCODE processed redacted alignments file {ENCFF489CFC|/files/ENCFF489CFC/}\ \ processed by ChIP-seq ENCODE4 v1.7.0 GRCh38 pipeline was generated from a library\ \ with PBC1 value of 0.77.\n\n**audit\\_warning**: PBC2 (PCR Bottlenecking Coefficient\ \ 2, M1/M2) is the ratio of the number of genomic locations where exactly one\ \ read maps uniquely (M1) to the number of genomic locations where two reads map\ \ uniquely (M2). A PBC2 value in the range 0 - 1 is severe bottlenecking, 1 -\ \ 3 is moderate bottlenecking, 3 - 10 is mild bottlenecking, > 10 is no bottlenecking.\ \ PBC2 value > 10 is recommended, but > 3 is acceptable. ENCODE processed redacted\ \ alignments file {ENCFF489CFC|/files/ENCFF489CFC/} processed by ChIP-seq ENCODE4\ \ v1.7.0 GRCh38 pipeline was generated from a library with PBC2 value of 4.08." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR580WRV status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens CD4-positive, alpha-beta memory T cell replication_type: anisogenic biosample_ontology: /biosample-types/primary_cell_CL_0000897/ perturbed: false doi: 10.17989/ENCSR580WRV date_created: '2015-09-15T19:49:03.446802+00:00' date_released: '2013-07-31' submitter_comment: '' target: H3K36me3