type: position_score table: filename: ENCFF584JOM.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR571IIS column_index: 3 type: float desc: signal histogram: type: number number_of_bins: 100 meta: summary: 'ChIP-seq ENCSR571IIS [TISSUE: Homo sapiens H1, TARGET: H2AFZ]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1\n\n**summary**:\ \ \n\n**output\\_type**: pseudoreplicated peaks\n\n**audit\\_internal\\_action**:\ \ Released analysis {ENCAN000OCY|/analyses/ENCAN000OCY/} has in progress subobject\ \ quality standard {encode4-histone-chip|/quality-standards/encode4-histone-chip/}\n\ \n**audit\\_not\\_compliant**: Processed alignments file {ENCFF897EWW|/files/ENCFF897EWW/}\ \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline has 7562856 usable fragments.\ \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H2AFZ-human and investigated as a narrow histone mark is 10 million usable fragments.\ \ The recommended value is > 20 million, but > 10 million is acceptable. (See\ \ {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\_not\\\ _compliant**: NRF (Non Redundant Fraction) is equal to the result of the division\ \ of the number of reads after duplicates removal by the total number of reads.\ \ An NRF value in the range 0 - 0.5 is poor complexity, 0.5 - 0.8 is moderate\ \ complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended, but >\ \ 0.5 is acceptable. ENCODE processed alignments file {ENCFF897EWW|/files/ENCFF897EWW/}\ \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\ \ with NRF value of 0.39.\n\n**audit\\_not\\_compliant**: PBC1 (PCR Bottlenecking\ \ Coefficient 1, M1/M\\_distinct) is the ratio of the number of genomic locations\ \ where exactly one read maps uniquely (M1) to the number of genomic locations\ \ where some reads map (M\\_distinct). A PBC1 value in the range 0 - 0.5 is severe\ \ bottlenecking, 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking,\ \ and > 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is\ \ acceptable. ENCODE processed alignments file {ENCFF897EWW|/files/ENCFF897EWW/}\ \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\ \ with PBC1 value of 0.45.\n\n**audit\\_warning**: PBC2 (PCR Bottlenecking Coefficient\ \ 2, M1/M2) is the ratio of the number of genomic locations where exactly one\ \ read maps uniquely (M1) to the number of genomic locations where two reads map\ \ uniquely (M2). A PBC2 value in the range 0 - 1 is severe bottlenecking, 1 -\ \ 3 is moderate bottlenecking, 3 - 10 is mild bottlenecking, > 10 is no bottlenecking.\ \ PBC2 value > 10 is recommended, but > 3 is acceptable. ENCODE processed alignments\ \ file {ENCFF897EWW|/files/ENCFF897EWW/} processed by ChIP-seq ENCODE4 v1.6.1\ \ GRCh38 pipeline was generated from a library with PBC2 value of 1.99." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR571IIS status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens H1 replication_type: unreplicated biosample_ontology: /biosample-types/cell_line_EFO_0003042/ perturbed: false doi: 10.17989/ENCSR571IIS date_created: '2015-10-01T07:16:23.743561+00:00' date_released: '2013-07-31' submitter_comment: '' target: H2AFZ