type: position_score table: filename: ENCFF918VFL.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR395USV column_index: 3 type: float desc: signal histogram: type: number number_of_bins: 100 meta: summary: 'ChIP-seq ENCSR395USV [TISSUE: Homo sapiens H1, TARGET: H3K9me3]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\ \n**summary**: \n\n**output\\_type**: pseudoreplicated peaks\n\n**audit\\_internal\\\ _action**: Released analysis {ENCAN176BQD|/analyses/ENCAN176BQD/} has in progress\ \ subobject quality standard {encode4-histone-chip|/quality-standards/encode4-histone-chip/}\n\ \n**audit\\_internal\\_action**: Released analysis {ENCAN176BQD|/analyses/ENCAN176BQD/}\ \ has in progress subobject document {00d78cd7-9a98-40b5-ae75-92f0673b7bd5|/documents/00d78cd7-9a98-40b5-ae75-92f0673b7bd5/}\n\ \n**audit\\_not\\_compliant**: Processed unfiltered alignments file {ENCFF868PKR|/files/ENCFF868PKR/}\ \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline has 27860858 mapped reads.\ \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K9me3-human and investigated as a broad histone mark is 35 million mapped\ \ reads. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _not\\_compliant**: Processed unfiltered alignments file {ENCFF118JTP|/files/ENCFF118JTP/}\ \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline has 24574444 mapped reads.\ \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K9me3-human and investigated as a broad histone mark is 35 million mapped\ \ reads. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _warning**: NRF (Non Redundant Fraction) is equal to the result of the division\ \ of the number of reads after duplicates removal by the total number of reads.\ \ An NRF value in the range 0 - 0.5 is poor complexity, 0.5 - 0.8 is moderate\ \ complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended, but >\ \ 0.5 is acceptable. ENCODE processed alignments file {ENCFF345MBH|/files/ENCFF345MBH/}\ \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\ \ with NRF value of 0.67.\n\n**audit\\_warning**: PBC1 (PCR Bottlenecking Coefficient\ \ 1, M1/M\\_distinct) is the ratio of the number of genomic locations where exactly\ \ one read maps uniquely (M1) to the number of genomic locations where some reads\ \ map (M\\_distinct). A PBC1 value in the range 0 - 0.5 is severe bottlenecking,\ \ 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking, and >\ \ 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is acceptable.\ \ ENCODE processed alignments file {ENCFF345MBH|/files/ENCFF345MBH/} processed\ \ by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library with\ \ PBC1 value of 0.68.\n\n**audit\\_warning**: PBC2 (PCR Bottlenecking Coefficient\ \ 2, M1/M2) is the ratio of the number of genomic locations where exactly one\ \ read maps uniquely (M1) to the number of genomic locations where two reads map\ \ uniquely (M2). A PBC2 value in the range 0 - 1 is severe bottlenecking, 1 -\ \ 3 is moderate bottlenecking, 3 - 10 is mild bottlenecking, > 10 is no bottlenecking.\ \ PBC2 value > 10 is recommended, but > 3 is acceptable. ENCODE processed alignments\ \ file {ENCFF345MBH|/files/ENCFF345MBH/} processed by ChIP-seq ENCODE4 v1.6.1\ \ GRCh38 pipeline was generated from a library with PBC2 value of 3.13." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR395USV status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens H1 replication_type: isogenic biosample_ontology: /biosample-types/cell_line_EFO_0003042/ perturbed: false doi: 10.17989/ENCSR395USV date_created: '2015-09-15T20:27:05.849442+00:00' date_released: '2013-07-31' submitter_comment: "This experiment\u2019s alignments have been cropped to 50nt\ \ for consistency against its control experiment." target: H3K9me3