type: position_score
table:
  filename: ENCFF992PIN.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: Histone_ChIP-seq_ENCSR248KRU
  column_index: 3
  type: float
  desc: 'Histone_ChIP-seq ENCSR248KRU [biosample_summary="Homo sapiens IMR-90" and target="H2BK5ac"]'
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'Histone_ChIP-seq ENCSR248KRU [biosample_summary="Homo sapiens IMR-90" and target="H2BK5ac"]'
  description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\
    \n**summary**: \n\n**output\\_type**: pseudoreplicated peaks\n\n**audit\\_internal\\\
    _action**: Released analysis {ENCAN359KLS|/analyses/ENCAN359KLS/} has in progress\
    \ subobject quality standard {encode4-histone-chip|/quality-standards/encode4-histone-chip/}\n\
    \n**audit\\_internal\\_action**: Released analysis {ENCAN359KLS|/analyses/ENCAN359KLS/}\
    \ has in progress subobject document {dd974fff-c248-4e23-9cd0-0b2a0a4f0c0b|/documents/dd974fff-c248-4e23-9cd0-0b2a0a4f0c0b/}\n\
    \n**audit\\_not\\_compliant**: NRF (Non Redundant Fraction) is equal to the result\
    \ of the division of the number of reads after duplicates removal by the total\
    \ number of reads. An NRF value in the range 0 - 0.5 is poor complexity, 0.5 -\
    \ 0.8 is moderate complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended,\
    \ but > 0.5 is acceptable. ENCODE processed alignments file {ENCFF895PTJ|/files/ENCFF895PTJ/}\
    \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\
    \ with NRF value of 0.45.\n\n**audit\\_not\\_compliant**: PBC1 (PCR Bottlenecking\
    \ Coefficient 1, M1/M\\_distinct) is the ratio of the number of genomic locations\
    \ where exactly one read maps uniquely (M1) to the number of genomic locations\
    \ where some reads map (M\\_distinct). A PBC1 value in the range 0 - 0.5 is severe\
    \ bottlenecking, 0.5 - 0.8 is moderate bottlenecking, 0.8 - 0.9 is mild bottlenecking,\
    \ and > 0.9 is no bottlenecking. PBC1 value > 0.9 is recommended, but > 0.8 is\
    \ acceptable. ENCODE processed alignments file {ENCFF895PTJ|/files/ENCFF895PTJ/}\
    \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\
    \ with PBC1 value of 0.50.\n\n**audit\\_warning**: Processed alignments file {ENCFF895PTJ|/files/ENCFF895PTJ/}\
    \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline has 19109075 usable fragments.\
    \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\
    \ H2BK5ac-human and investigated as a transcription factor is 10 million usable\
    \ fragments. The recommended value is > 20 million, but > 10 million is acceptable.\
    \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/} )\n\n**audit\\\
    _warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio of the\
    \ number of genomic locations where exactly one read maps uniquely (M1) to the\
    \ number of genomic locations where two reads map uniquely (M2). A PBC2 value\
    \ in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking,\
    \ 3 - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,\
    \ but > 3 is acceptable. ENCODE processed alignments file {ENCFF895PTJ|/files/ENCFF895PTJ/}\
    \ processed by ChIP-seq ENCODE4 v1.6.1 GRCh38 pipeline was generated from a library\
    \ with PBC2 value of 2.22."
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR248KRU
    status: released
    assay_term_name: ChIP-seq
    simple_biosample_summary: ''
    biosample_summary: Homo sapiens IMR-90
    replication_type: isogenic
    biosample_ontology: /biosample-types/cell_line_EFO_0001196/
    perturbed: false
    doi: 10.17989/ENCSR248KRU
    date_created: '2015-10-01T07:16:31.035113+00:00'
    date_released: '2013-07-31'
    submitter_comment: ''
    target: H2BK5ac