type: position_score table: filename: ENCFF803FDJ.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR236YPE column_index: 3 type: float desc: signal histogram: type: number number_of_bins: 100 meta: summary: 'ChIP-seq ENCSR236YPE [TISSUE: Homo sapiens GM23338 originated from GM23248, TARGET: H3K36me3]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\ \n**summary**: \n\n**output\\_type**: pseudoreplicated peaks\n\n**audit\\_internal\\\ _action**: Released analysis {ENCAN116HRR|/analyses/ENCAN116HRR/} has in progress\ \ subobject quality standard {encode4-histone-chip|/quality-standards/encode4-histone-chip/}\n\ \n**audit\\_not\\_compliant**: Processed alignments file {ENCFF305DFQ|/files/ENCFF305DFQ/}\ \ processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline has 18687998 usable fragments.\ \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K36me3-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _not\\_compliant**: Processed alignments file {ENCFF969XFC|/files/ENCFF969XFC/}\ \ processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline has 17230273 usable fragments.\ \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K36me3-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio of the\ \ number of genomic locations where exactly one read maps uniquely (M1) to the\ \ number of genomic locations where two reads map uniquely (M2). A PBC2 value\ \ in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking,\ \ 3 - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,\ \ but > 3 is acceptable. ENCODE processed alignments file {ENCFF305DFQ|/files/ENCFF305DFQ/}\ \ processed by ChIP-seq ENCODE4 v1.8.0 GRCh38 pipeline was generated from a library\ \ with PBC2 value of 9.89." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR236YPE status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens GM23338 originated from GM23248 replication_type: isogenic biosample_ontology: /biosample-types/cell_line_EFO_0007950/ perturbed: false doi: 10.17989/ENCSR236YPE date_created: '2015-08-27T17:08:16.141888+00:00' date_released: '2016-05-03' submitter_comment: Files ENCFF483QVH, ENCFF562IES had incorrectly identified paired_end 1 and 2 metadata respectively. They have been corrected to match its fastq file flowcell signature accordingly. target: H3K36me3