type: position_score table: filename: ENCFF202WFY.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR125IJY column_index: 3 type: float desc: 'Histone_ChIP-seq ENCSR125IJY [biosample_summary="Homo sapiens SK-N-SH" and target="H3F3A"]' histogram: type: number number_of_bins: 100 meta: summary: 'Histone_ChIP-seq ENCSR125IJY [biosample_summary="Homo sapiens SK-N-SH" and target="H3F3A"]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\ \n**summary**: \n\n**output\\_type**: replicated peaks\n\n**audit\\_internal\\\ _action**: Archived analysis {ENCAN291VUG|/analyses/ENCAN291VUG/} has in progress\ \ subobject quality standard {encode3-histone-chip|/quality-standards/encode3-histone-chip/}\n\ \n**audit\\_not\\_compliant**: Processed alignments file {ENCFF754AJD|/files/ENCFF754AJD/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline has 22897336 usable fragments. The\ \ minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3F3A-human and investigated as a broad histone mark is 20 million usable fragments.\ \ The recommended value is > 45 million, but > 35 million is acceptable. (See\ \ {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\_not\\\ _compliant**: Processed alignments file {ENCFF671ZSB|/files/ENCFF671ZSB/} processed\ \ by ChIP-seq ENCODE3 hg19 pipeline has 22504933 usable fragments. The minimum\ \ ENCODE standard for each replicate in a ChIP-seq experiment targeting H3F3A-human\ \ and investigated as a broad histone mark is 20 million usable fragments. The\ \ recommended value is > 45 million, but > 35 million is acceptable. (See {ENCODE\ \ ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\_warning**:\ \ PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio of the number of\ \ genomic locations where exactly one read maps uniquely (M1) to the number of\ \ genomic locations where two reads map uniquely (M2). A PBC2 value in the range\ \ 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3 - 10 is mild\ \ bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended, but\ \ > 3 is acceptable. ENCODE processed alignments file {ENCFF671ZSB|/files/ENCFF671ZSB/}\ \ processed by ChIP-seq ENCODE3 hg19 pipeline was generated from a library with\ \ PBC2 value of 9.93." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR125IJY status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens SK-N-SH replication_type: isogenic biosample_ontology: /biosample-types/cell_line_EFO_0003072/ perturbed: false doi: 10.17989/ENCSR125IJY date_created: '2015-05-20T20:37:38.800494+00:00' date_released: '2016-10-19' submitter_comment: '' target: H3F3A