type: position_score
table:
  filename: ENCFF686GBZ.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: Histone_ChIP-seq_ENCSR000DWU
  column_index: 3
  type: float
  desc: 'Histone_ChIP-seq ENCSR000DWU [biosample_summary="Homo sapiens keratinocyte female" and target="H3K27me3"]'
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'Histone_ChIP-seq ENCSR000DWU [biosample_summary="Homo sapiens keratinocyte female" and target="H3K27me3"]'
  description: '**status**: released


    **biological\_replicates**: Rep 1, Rep 2


    **summary**: female


    **output\_type**: pseudoreplicated peaks


    **audit\_error**: Processed alignments file {ENCFF838LHK|/files/ENCFF838LHK/}
    processed by ChIP-seq ENCODE4 v1.6.0 GRCh38 pipeline has 4902202 usable fragments.
    The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting
    H3K27me3-human and investigated as a broad histone mark is 20 million usable fragments.
    The recommended value is > 45 million, but > 35 million is acceptable. (See {ENCODE
    ChIP-seq data standards|/data-standards/chip-seq/})


    **audit\_error**: Processed alignments file {ENCFF607YOM|/files/ENCFF607YOM/}
    processed by ChIP-seq ENCODE4 v1.6.0 GRCh38 pipeline has 4369822 usable fragments.
    The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting
    H3K27me3-human and investigated as a broad histone mark is 20 million usable fragments.
    The recommended value is > 45 million, but > 35 million is acceptable. (See {ENCODE
    ChIP-seq data standards|/data-standards/chip-seq/})


    **audit\_internal\_action**: Released analysis {ENCAN486JPT|/analyses/ENCAN486JPT/}
    has in progress subobject quality standard {encode4-histone-chip|/quality-standards/encode4-histone-chip/}


    **audit\_internal\_action**: Released analysis {ENCAN486JPT|/analyses/ENCAN486JPT/}
    has in progress subobject document {a34b7ffc-257e-4f2e-8ded-74ad914fc7d3|/documents/a34b7ffc-257e-4f2e-8ded-74ad914fc7d3/}


    **audit\_not\_compliant**: NRF (Non Redundant Fraction) is equal to the result
    of the division of the number of reads after duplicates removal by the total number
    of reads. An NRF value in the range 0 - 0.5 is poor complexity, 0.5 - 0.8 is moderate
    complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended, but > 0.5
    is acceptable. ENCODE processed alignments file {ENCFF838LHK|/files/ENCFF838LHK/}
    processed by ChIP-seq ENCODE4 v1.6.0 GRCh38 pipeline was generated from a library
    with NRF value of 0.27.


    **audit\_not\_compliant**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\_distinct)
    is the ratio of the number of genomic locations where exactly one read maps uniquely
    (M1) to the number of genomic locations where some reads map (M\_distinct). A
    PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate
    bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking.
    PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments
    file {ENCFF838LHK|/files/ENCFF838LHK/} processed by ChIP-seq ENCODE4 v1.6.0 GRCh38
    pipeline was generated from a library with PBC1 value of 0.19.


    **audit\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio
    of the number of genomic locations where exactly one read maps uniquely (M1) to
    the number of genomic locations where two reads map uniquely (M2). A PBC2 value
    in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3
    - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,
    but > 3 is acceptable. ENCODE processed alignments file {ENCFF838LHK|/files/ENCFF838LHK/}
    processed by ChIP-seq ENCODE4 v1.6.0 GRCh38 pipeline was generated from a library
    with PBC2 value of 1.03.


    **audit\_warning**: NRF (Non Redundant Fraction) is equal to the result of the
    division of the number of reads after duplicates removal by the total number of
    reads. An NRF value in the range 0 - 0.5 is poor complexity, 0.5 - 0.8 is moderate
    complexity, and > 0.8 high complexity. NRF value > 0.8 is recommended, but > 0.5
    is acceptable. ENCODE processed alignments file {ENCFF607YOM|/files/ENCFF607YOM/}
    processed by ChIP-seq ENCODE4 v1.6.0 GRCh38 pipeline was generated from a library
    with NRF value of 0.62.


    **audit\_warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\_distinct) is
    the ratio of the number of genomic locations where exactly one read maps uniquely
    (M1) to the number of genomic locations where some reads map (M\_distinct). A
    PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate
    bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking.
    PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments
    file {ENCFF607YOM|/files/ENCFF607YOM/} processed by ChIP-seq ENCODE4 v1.6.0 GRCh38
    pipeline was generated from a library with PBC1 value of 0.58.


    **audit\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio
    of the number of genomic locations where exactly one read maps uniquely (M1) to
    the number of genomic locations where two reads map uniquely (M2). A PBC2 value
    in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3
    - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,
    but > 3 is acceptable. ENCODE processed alignments file {ENCFF607YOM|/files/ENCFF607YOM/}
    processed by ChIP-seq ENCODE4 v1.6.0 GRCh38 pipeline was generated from a library
    with PBC2 value of 2.16.'
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR000DWU
    status: released
    assay_term_name: ChIP-seq
    simple_biosample_summary: female
    biosample_summary: Homo sapiens keratinocyte female
    replication_type: isogenic
    biosample_ontology: /biosample-types/primary_cell_CL_0000312/
    perturbed: false
    doi: 10.17989/ENCSR000DWU
    date_created: '2014-02-12T22:47:30.076124+00:00'
    date_released: '2011-02-11'
    submitter_comment: Replicate 2,1 of this experiment was previously attributed
      to biosample ENCBS591ENC and replicate 1,1 of this experiment was previously
      attributed to biosample ENCBS591ENC but they have since been corrected to the
      current biosamples. Please note that the biosample term has remain unchanged.
    target: H3K27me3