type: position_score table: filename: ENCFF662NTZ.tabix.bed.gz header_mode: none zero_based: true format: tabix chrom: column_index: 0 pos_begin: column_index: 1 pos_end: column_index: 2 scores: - id: Histone_ChIP-seq_ENCSR000DST column_index: 3 type: float desc: 'Histone_ChIP-seq ENCSR000DST [biosample_summary="Homo sapiens H7" and target="H3K27me3"]' histogram: type: number number_of_bins: 100 meta: summary: 'Histone_ChIP-seq ENCSR000DST [biosample_summary="Homo sapiens H7" and target="H3K27me3"]' description: "**status**: released\n\n**biological\\_replicates**: Rep 1, Rep 2\n\ \n**summary**: \n\n**output\\_type**: pseudoreplicated peaks\n\n**audit\\_internal\\\ _action**: Released analysis {ENCAN621OIG|/analyses/ENCAN621OIG/} has in progress\ \ subobject quality standard {encode4-histone-chip|/quality-standards/encode4-histone-chip/}\n\ \n**audit\\_internal\\_action**: Released analysis {ENCAN621OIG|/analyses/ENCAN621OIG/}\ \ has in progress subobject document {d3ae894c-bb95-4fba-a1d2-afc550e8f507|/documents/d3ae894c-bb95-4fba-a1d2-afc550e8f507/}\n\ \n**audit\\_not\\_compliant**: Processed alignments file {ENCFF198VKK|/files/ENCFF198VKK/}\ \ processed by ChIP-seq ENCODE4 v1.6.0 GRCh38 pipeline has 9837107 usable fragments.\ \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K27me3-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _not\\_compliant**: Processed alignments file {ENCFF250SOU|/files/ENCFF250SOU/}\ \ processed by ChIP-seq ENCODE4 v1.6.0 GRCh38 pipeline has 16969020 usable fragments.\ \ The minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting\ \ H3K27me3-human and investigated as a broad histone mark is 20 million usable\ \ fragments. The recommended value is > 45 million, but > 35 million is acceptable.\ \ (See {ENCODE ChIP-seq data standards|/data-standards/chip-seq/})\n\n**audit\\\ _warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\\_distinct) is the ratio\ \ of the number of genomic locations where exactly one read maps uniquely (M1)\ \ to the number of genomic locations where some reads map (M\\_distinct). A PBC1\ \ value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate bottlenecking,\ \ 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking. PBC1 value >\ \ 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments file\ \ {ENCFF250SOU|/files/ENCFF250SOU/} processed by ChIP-seq ENCODE4 v1.6.0 GRCh38\ \ pipeline was generated from a library with PBC1 value of 0.90.\n\n**audit\\\ _warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio of the\ \ number of genomic locations where exactly one read maps uniquely (M1) to the\ \ number of genomic locations where two reads map uniquely (M2). A PBC2 value\ \ in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking,\ \ 3 - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,\ \ but > 3 is acceptable. ENCODE processed alignments file {ENCFF250SOU|/files/ENCFF250SOU/}\ \ processed by ChIP-seq ENCODE4 v1.6.0 GRCh38 pipeline was generated from a library\ \ with PBC2 value of 9.91." labels: reference_genome: hg38/genomes/GRCh38-hg38 accession: ENCSR000DST status: released assay_term_name: ChIP-seq simple_biosample_summary: '' biosample_summary: Homo sapiens H7 replication_type: isogenic biosample_ontology: /biosample-types/cell_line_EFO_0005904/ perturbed: false doi: 10.17989/ENCSR000DST date_created: '2013-12-12T06:57:18.532753+00:00' date_released: '2011-02-11' submitter_comment: Replicate 1,1 of this experiment was previously attributed to biosample ENCBS293AAA and replicate 2,1 of this experiment was previously attributed to biosample ENCBS293AAA but they have since been corrected to the current biosamples. Please note that the biosample term has remain unchanged. target: H3K27me3