type: position_score
table:
  filename: ENCFF258BVG.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: Histone_ChIP-seq_ENCSR000DQP
  column_index: 3
  type: float
  desc: 'Histone_ChIP-seq ENCSR000DQP [biosample_summary="Homo sapiens B cell female adult (43 years)" and target="H3K4me3"]'
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'Histone_ChIP-seq ENCSR000DQP [biosample_summary="Homo sapiens B cell female adult (43 years)" and target="H3K4me3"]'
  description: '**status**: released


    **biological\_replicates**: Rep 1, Rep 2


    **summary**: female adult (43 years)


    **output\_type**: replicated peaks


    **audit\_internal\_action**: Archived analysis {ENCAN279TAZ|/analyses/ENCAN279TAZ/}
    has in progress subobject quality standard {encode3-histone-chip|/quality-standards/encode3-histone-chip/}


    **audit\_warning**: Processed alignments file {ENCFF642FEV|/files/ENCFF642FEV/}
    processed by ChIP-seq ENCODE3 GRCh38 pipeline has 14344681 usable fragments. The
    minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting
    H3K4me3-human and investigated as a narrow histone mark is 10 million usable fragments.
    The recommended value is > 20 million, but > 10 million is acceptable. (See {ENCODE
    ChIP-seq data standards|/data-standards/chip-seq/})


    **audit\_warning**: Processed alignments file {ENCFF842FEQ|/files/ENCFF842FEQ/}
    processed by ChIP-seq ENCODE3 GRCh38 pipeline has 17755740 usable fragments. The
    minimum ENCODE standard for each replicate in a ChIP-seq experiment targeting
    H3K4me3-human and investigated as a narrow histone mark is 10 million usable fragments.
    The recommended value is > 20 million, but > 10 million is acceptable. (See {ENCODE
    ChIP-seq data standards|/data-standards/chip-seq/})


    **audit\_warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\_distinct) is
    the ratio of the number of genomic locations where exactly one read maps uniquely
    (M1) to the number of genomic locations where some reads map (M\_distinct). A
    PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate
    bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking.
    PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments
    file {ENCFF642FEV|/files/ENCFF642FEV/} processed by ChIP-seq ENCODE3 GRCh38 pipeline
    was generated from a library with PBC1 value of 0.86.


    **audit\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio
    of the number of genomic locations where exactly one read maps uniquely (M1) to
    the number of genomic locations where two reads map uniquely (M2). A PBC2 value
    in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3
    - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,
    but > 3 is acceptable. ENCODE processed alignments file {ENCFF642FEV|/files/ENCFF642FEV/}
    processed by ChIP-seq ENCODE3 GRCh38 pipeline was generated from a library with
    PBC2 value of 7.64.


    **audit\_warning**: PBC1 (PCR Bottlenecking Coefficient 1, M1/M\_distinct) is
    the ratio of the number of genomic locations where exactly one read maps uniquely
    (M1) to the number of genomic locations where some reads map (M\_distinct). A
    PBC1 value in the range 0 - 0.5 is severe bottlenecking, 0.5 - 0.8 is moderate
    bottlenecking, 0.8 - 0.9 is mild bottlenecking, and > 0.9 is no bottlenecking.
    PBC1 value > 0.9 is recommended, but > 0.8 is acceptable. ENCODE processed alignments
    file {ENCFF842FEQ|/files/ENCFF842FEQ/} processed by ChIP-seq ENCODE3 GRCh38 pipeline
    was generated from a library with PBC1 value of 0.83.


    **audit\_warning**: PBC2 (PCR Bottlenecking Coefficient 2, M1/M2) is the ratio
    of the number of genomic locations where exactly one read maps uniquely (M1) to
    the number of genomic locations where two reads map uniquely (M2). A PBC2 value
    in the range 0 - 1 is severe bottlenecking, 1 - 3 is moderate bottlenecking, 3
    - 10 is mild bottlenecking, > 10 is no bottlenecking. PBC2 value > 10 is recommended,
    but > 3 is acceptable. ENCODE processed alignments file {ENCFF842FEQ|/files/ENCFF842FEQ/}
    processed by ChIP-seq ENCODE3 GRCh38 pipeline was generated from a library with
    PBC2 value of 6.70.'
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR000DQP
    status: released
    assay_term_name: ChIP-seq
    simple_biosample_summary: female adult (43 years)
    biosample_summary: Homo sapiens B cell female adult (43 years)
    replication_type: isogenic
    biosample_ontology: /biosample-types/primary_cell_CL_0000236/
    perturbed: false
    doi: 10.17989/ENCSR000DQP
    date_created: '2014-02-12T22:50:12.414206+00:00'
    date_released: '2011-11-17'
    submitter_comment: Replicate 2,1 of this experiment was previously attributed
      to biosample ENCBS483ENC and replicate 1,1 of this experiment was previously
      attributed to biosample ENCBS483ENC but they have since been corrected to the
      current biosamples. Please note that the biosample term has remain unchanged.
    target: H3K4me3