type: position_score
table:
  filename: ENCFF199PMO.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: DNase-seq_ENCSR175OHT
  column_index: 3
  type: float
  desc: signal
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'DNase-seq ENCSR175OHT [TISSUE: Homo sapiens activated CD8-positive, alpha-beta
    memory T cell male adult (38 years) treated with anti-CD3 and anti-CD28 coated
    beads for 24 hours, 50 U/mL Interleukin-2 for 24 hours]'
  description: '**status**: released


    **biological\_replicates**: Rep 1


    **summary**: male adult (38 years) treated with anti-CD3 and anti-CD28 coated
    beads for 24 hours, 50 U/mL Interleukin-2 for 24 hours


    **output\_type**: peaks


    **audit\_error**: Alignment file {ENCFF782CFI|/files/ENCFF782CFI/} processed by
    DNase-seq ENCODE4 v3.0.0-alpha.2 GRCh38 pipeline ( {ENCPL848KLD|/pipelines/ENCPL848KLD/}
    ) for GRCh38 assembly has 16071086 mapped reads. According to ENCODE standards,
    conventional DNase-seq profile requires a minimum of 20 million uniquely mapped
    reads to generate a reliable SPOT (Signal Portion of Tags) score. The recommended
    value is > 50 million. For deep, foot-printing depth DNase-seq 150-200 million
    uniquely mapped reads are recommended. (See {ENCODE DNase-seq data standards|/data-standards/dnase-seq-encode4/})


    **audit\_internal\_action**: Released analysis {ENCAN694QOH|/analyses/ENCAN694QOH/}
    has in progress subobject document {edc616f9-f928-46f0-bcae-20cf7654fcc6|/documents/edc616f9-f928-46f0-bcae-20cf7654fcc6/}


    **audit\_internal\_action**: Released analysis {ENCAN694QOH|/analyses/ENCAN694QOH/}
    has in progress subobject document {3a20fd08-9f0c-4883-84f6-c8f1a5eb3edb|/documents/3a20fd08-9f0c-4883-84f6-c8f1a5eb3edb/}'
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR175OHT
    status: released
    assay_term_name: DNase-seq
    simple_biosample_summary: male adult (38 years) treated with anti-CD3 and anti-CD28
      coated beads for 24 hours, 50 U/mL Interleukin-2 for 24 hours
    biosample_summary: Homo sapiens activated CD8-positive, alpha-beta memory T cell
      male adult (38 years) treated with anti-CD3 and anti-CD28 coated beads for 24
      hours, 50 U/mL Interleukin-2 for 24 hours
    replication_type: unreplicated
    biosample_ontology: /biosample-types/primary_cell_NTR_0000504/
    perturbed: false
    doi: 10.17989/ENCSR175OHT
    date_created: '2020-04-20T23:45:27.292873+00:00'
    date_released: '2020-09-28'
    submitter_comment: ''