type: position_score
table:
  filename: ENCFF257BSS.tabix.bed.gz
  header_mode: none
  zero_based: true
  format: tabix
  chrom:
    column_index: 0
  pos_begin:
    column_index: 1
  pos_end:
    column_index: 2
scores:
- id: DNase-seq_ENCSR133QIE
  column_index: 3
  type: float
  desc: signal
  histogram:
    type: number
    number_of_bins: 100
meta:
  summary: 'DNase-seq ENCSR133QIE [TISSUE: Homo sapiens stimulated activated effector
    memory CD8-positive, alpha-beta T cell male adult (33 years) treated with 100
    ng/mL Interleukin-12 subunit alpha for 72 hours, anti-CD3 and anti-CD28 coated
    beads for 72 hours, 100 ng/mL Interleukin-12 subunit beta for 72 hours]'
  description: '**status**: released


    **biological\_replicates**: Rep 1


    **summary**: male adult (33 years) treated with 100 ng/mL Interleukin-12 subunit
    alpha for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours, 100 ng/mL
    Interleukin-12 subunit beta for 72 hours


    **output\_type**: peaks


    **audit\_warning**: Signal Portion of Tags (SPOT) is a measure of enrichment,
    analogous to the commonly used fraction of reads in peaks metric. ENCODE processed
    alignment files {ENCFF192RAV|/files/ENCFF192RAV/} processed by DNase-seq ENCODE4
    v3.0.0 GRCh38 pipeline ({ENCPL848KLD|/pipelines/ENCPL848KLD/}) for GRCh38 assembly  have
    a SPOT1 score of 0.29. According to ENCODE standards, SPOT1 score of 0.4 or higher
    is considered a product of high quality data. Any sample with a SPOT1 score <0.3
    should be targeted for replacement with a higher quality sample, and a SPOT1 score
    of 0.25 is considered minimally acceptable SPOT1 score of 0.25 is considered minimally
    acceptable for rare and hard to find primary tissues. (See {ENCODE DNase-seq data
    standards|/data-standards/dnase-seq-encode4/})'
  labels:
    reference_genome: hg38/genomes/GRCh38-hg38
    accession: ENCSR133QIE
    status: released
    assay_term_name: DNase-seq
    simple_biosample_summary: male adult (33 years) treated with 100 ng/mL Interleukin-12
      subunit alpha for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours,
      100 ng/mL Interleukin-12 subunit beta for 72 hours
    biosample_summary: Homo sapiens stimulated activated effector memory CD8-positive,
      alpha-beta T cell male adult (33 years) treated with 100 ng/mL Interleukin-12
      subunit alpha for 72 hours, anti-CD3 and anti-CD28 coated beads for 72 hours,
      100 ng/mL Interleukin-12 subunit beta for 72 hours
    replication_type: unreplicated
    biosample_ontology: /biosample-types/primary_cell_NTR_0000668/
    perturbed: false
    doi: 10.17989/ENCSR133QIE
    date_created: '2021-08-23T23:11:18.255174+00:00'
    date_released: '2021-09-02'
    submitter_comment: ''